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زیست شناسی::
ناقل پلاسمیدی
The yeast episomal plasmid vectors are based on the highcopynumber 2µm plasmid, a small, circular plasmid found in most natural strains of
For example, when a plasmid vector with a wildtype LEU2 gene is transformed into a mutant leu2 host cell and plated onto medium that lacks leucine, only cells that carry the plasmid will grow.
form of the protein hirudin was synthesized and secreted by an S. cerevisiae strain containing a plasmid vector that had the preproafactor sequence added to the hirudin coding sequence.
When protein disulfide isomerase overproducing cells were transformed with a plasmid vector carrying the gene for human plateletderived growth factor B, there was a 10fold in crease in the secretion of recombinant protein over that of transformed cells with normal levels of protein disulfide isomerase.
Expression of high levels of protein from plasmid vectors is transient and inevitably results in loss of the vector, which cannot be propagated in mammalian cells, or death of the host cell.
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